Enumeration and Identification
PRINCIPLE OF THE TEST:
Selective enumeration by conventional viable cell count method:
The conventional bacterial viable cell count method relies on the accuracy in using suitable media and plating techniques. Most probiotic strains are facultative anaerobes or strict anaerobes. They require 36-48 hours to grow in a complex nutritional media. Aseptically, samples are dissolved in a sterile diluent and serial decimal dilutions are performed. Aliquots of selected dilutions are plated on appropriate media. The plates are incubated at 37oC for 48 hours. The colony forming unit (CFU) are counted.
Microbial Identification using the Eurofins IDmyk Comparative Sequencing system:
Unknown bacteria samples are identified using the Eurofins IDmyk Comparative Sequencing microbial identification software and 16S rRNA gene sequence database, containing over 8,470 entries. Routine bacterial identification is performed using the first 1,100 bp of the rDNA.